Chromatography Services Laboratory

Instrumental Analytical Services

The Chromatography Services Laboratory offers a number of Gas Liquid Chromatographs and High Performance Liquid Chromatographs to suit semi-preparative or analytical separation of chemical compounds. The chromatographic runs are performed by the research analyst with the assistance of the lab manager, Mr Victor De Guzman, who can provide advice on instrument operation, hands-on training and safety.

This service is provided to the School of Chemistry, Centre for Green Chemistry, other Monash Faculties and external commercial clients. Charges for chromatographic analysis are negotiated on an individual basis. Priority access is given to research staff and post-graduate students during school terms. The facility provides generic columns for routine analytical applications however, in some cases, specialized columns for non-routine chromatographic applications need to be supplied by the analyst.

School of Chemistry, Clayton Campus
Location: Bldg 23, room 48
Phone: +613 9905 4584
Fax: +613 9905 4597
Mobile: 0411 703 802

Gas Chromatographs (GLC)

Legend: FID = Flame Ionization Detector; TCD = Thermal Conductivity Detector

Code	Brand & Model	Detector	Column Type	Application
GLC 1	Varian 3700 Manual Injection	FID	A: 6’ X ¼” 10% SE30 B: 30mx0.25mmx0.12um BPX5 (SGE)	A: Pack column for neat samples B: Capillary column for trace analysis of non polar compounds
GLC 2	HP 5890 Manual Injection	FID	50mx0.25mmx0.12um e.g.XE-60-S-VAL-S-A-PEA	Trace analysis of chiral compounds (Supply own column)
GLC 3	Varian 3700 Manual Injection	FID	A: 12QC5 BP20 B: 6’x1/4” 10% Carbowax 20M on AWDMCS	A: Capillary column for trace analysis of polar compounds B: Pack column for neat polar samples
GLC 4	Varian 3700 Manual Valve Injection	TCD / FID	A: 6’x1/4: Poropak Q / Molecular sieve B: C-18 Porasil	A: Pack column for gas analysis of permanent gases. B: Pack column for Hydrocarbons (C1-C5)
GLC 5	Agilent 6850 Auto Sampler	FID	30mx0.32mmx0.25um J&W HP-1	Capillary column for trace analysis of non polar compounds to intermediate compounds
GLC 6	Agilent 6850 Auto Sampler	FID	30mx0.32mmx0.25um J&W HP-1	Capillary column for trace analysis of non polar compounds to intermediate compounds
GLC 7	Shimadzu GC8A Manual Injection	FID	50m x0.53mm CP AL2O3 / KCL	Capillary column for trace analysis of volatile compounds

High Performance Liquid Chromatographs (HPLC)

Legend: ELSD = Evaporative Light Scattering Detector, PDA = Photo Diode Detector, UV = Ultraviolet detector

Code

Brand & Model

Detector

Column Type

Application

LC 1

Agilent 1200 RR Auto sampler

PDA

4mmx150mm 5um
ZORBAX ECLIPSE C18

Reverse phase rapid resolution separations or Normal phase chiral separations
(Supply own column)

LC 2

Waters 6000A
Manual injection

Variable UV/VIS

Semi-preparative or analytical reverse phase columns

Reverse phase in binary gradient elution or
Isocratic runs with a variable UV wavelength
selector

LC 3

Waters 600
Auto sampler

PDA

Reverse phase separation in analytical mode

Reverse phase in Quarternary gradient elution
or Isocratic runs with PDA and /or
ELSD detector

LC 4

Agilent 1200 Auto sampler

PDA

Reverse phase separation in analytical mode

Reverse phase in Quarternary gradient elution or Isocratic runs with PDA detector

LC 5

Agilent 1200 Auto sampler

PDA / ELSD

Reverse phase or GPC separation in analytical mode

Reverse phase in quaternary gradient elution or Isocratic Size exclusion runs with PDA detector and ELSD.